Part:BBa_J61031:Design

Amplifiable BAC plasmid

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 6527
  Illegal SpeI site found at 2
  Illegal PstI site found at 2001
  Illegal PstI site found at 3542
  Illegal PstI site found at 5068
  Illegal PstI site found at 8975
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 6527
  Illegal NheI site found at 8816
  Illegal SpeI site found at 2
  Illegal PstI site found at 2001
  Illegal PstI site found at 3542
  Illegal PstI site found at 5068
  Illegal PstI site found at 8975
  Illegal NotI site found at 9
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 6527
  Illegal BglII site found at 2350
  Illegal BglII site found at 4421
  Illegal BglII site found at 4855
  Illegal XhoI site found at 4554
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 6527
  Illegal SpeI site found at 2
  Illegal PstI site found at 2001
  Illegal PstI site found at 3542
  Illegal PstI site found at 5068
  Illegal PstI site found at 8975
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 6527
  Illegal SpeI site found at 2
  Illegal PstI site found at 2001
  Illegal PstI site found at 3542
  Illegal PstI site found at 5068
  Illegal PstI site found at 8975
  Illegal NgoMIV site found at 3860
  Illegal NgoMIV site found at 5519
  Illegal AgeI site found at 1613
  Illegal AgeI site found at 2506
  Illegal AgeI site found at 3736
  Illegal AgeI site found at 10369
  Illegal AgeI site found at 10481
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI site found at 7384
  Illegal BsaI site found at 9050
  Illegal BsaI.rc site found at 759
  Illegal BsaI.rc site found at 5690
  Illegal BsaI.rc site found at 6256
  Illegal BsaI.rc site found at 6434
  Illegal SapI.rc site found at 1756
  Illegal SapI.rc site found at 2966
  Illegal SapI.rc site found at 5368
  Illegal SapI.rc site found at 5578
  Illegal SapI.rc site found at 8466
  

Design Notes

N/A

Source

many many steps

References

<biblio>

1. Hohn-Gene-1980 pmid=6260575
2. Ogura-Cell-1983 pmid=6297791
3. Abeles-JMB-1985 pmid=3903163
4. OConnor-Science-1989 pmid=2660262
5. Evans-Gene-1989 pmid=2777090
6. Shizuya-PNAS-1992 pmid=1528894
7. Kim-NAR-1992 pmid=1549470
8. Whipple-NAR-1998 pmid=9685485 (from Natalie)
9. Frengen-Genomics-1999 pmid=10373322
10. Wild-Genome-Res-2002 pmid=12213781
11. Casali-Ecoli-plasmids-2003 isbn=1-58829-151-0
12. Zhao-BAC-2004-v1 isbn=0-89603-988-9
13. Zhao-BAC-2004-v2 isbn=0-89603-989-7

</biblio>